Western Blot:Article Title: ALKBH1 promotes HIF-1 α -mediated glycolysis by inhibiting N- glycosylation of LAMP2A
Article Snippet: .. Antibody Name (Primary antibodies) Company Item number Application ALKBH1 Polyclonal antibody Proteintech 27973-1-AP WB, 1:2000 ALKBH1 Rabbit pAb ABclonal A14079 IP, 1:500 HIF-1α Rabbit mAb CST #36169S WB, 1:1000 Hexokinase 2 Polyclonal antibody Proteintech 22029-1-AP WB, 1:3000 PKM2-specific Polyclonal antibody Proteintech 15822-1-AP WB, 1:3000 PGK1 Polyclonal antibody Proteintech 17811-1-AP WB, 1:3000 LDHA-Specific Polyclonal antibody Proteintech 19987-1-AP WB, 1:3000 β-Actin Mouse monoclonal Sigma A1978 WB, 1:4000 α-Tubulin Rabbit mAb CST #2125 WB, 1:3000 LC3B antibody Sigma L7543 WB, 1:2000 p62/SQSTM1 antibody Sigma P0067 WB, 1:3000 Monoclonal ANTI-FLAG® M2 Sigma F1804 IP, 1:1000 LAMP2A Abcam ab125068 WB, 1:1000 LAMP1 Rabbit mAb CST #9091 WB, 1:3000 IF, 1:200 Hsc70 Polyclonal antibody Protientech 10654-1-AP WB, 1:3000 Anti-O-Linked N-Acetylglucosamine Rabbit mAb PTM BIO PTM-951RM IP, 1:1000 Galectin-3 Monoclonal antibody Protientech 60207-1-Ig IF, 1:200 MYC tag Polyclonal antibody Protientech 16286-1-AP IP, 1:1000 DDOST/OST48 Polyclonal antibody Protientech 14916-1-AP IP, 1:2000 STT3A Polyclonal antibody Protientech 12034-1-AP IP, 1:2000 DAD1 Polyclonal antibody Protientech 10531-1-AP IP, 1:2000 LRRK2 Rabbit pAb ABclonal A17253 WB, 1:2000 MEF2D Polyclonal antibody Protientech 14353-1-AP WB, 1:2000 Cathepsin B Polyclonal antibody Protientech 12216-1-AP WB, 1:2000 Cathepsin D Polyclonal antibody Protientech 55021-1-AP WB, 1:2000 TFEB Rabbit pAb ABclonal A7311 WB, 1:2000 Antibody Name (Secondary antibodies) Company Item number Application Peroxidase AffiniPure Goat Anti-Rabbit IgG Jackson ImmunoResearch 111-035-144 WB, 1:5000- 1:10000 Peroxidase AffiniPure Goat Anti-Mouse IgG Jackson ImmunoResearch 115-035-146 WB, 1:5000- 1:10000 Alexa Fluor®594-AffiniPure goat anti-rabbit IgG Jackson ImmunoResearch 115-585-146 IF, 1:200-400 Alexa Fluor®488-AffiniPure goat anti-mouse IgG Jackson ImmunoResearch 115-545-146 IF, 1:200-400 Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) CST #93702 IP, 1:2000 VeriBlot for IP Detection Reagent (HRP) Abcam ab131366 IP, 1:2000 Supplementary Table 2 qPCR primers sequences used in the present study. ..
Real-time Polymerase Chain Reaction:Article Title: ALKBH1 promotes HIF-1 α -mediated glycolysis by inhibiting N- glycosylation of LAMP2A
Article Snippet: .. Antibody Name (Primary antibodies) Company Item number Application ALKBH1 Polyclonal antibody Proteintech 27973-1-AP WB, 1:2000 ALKBH1 Rabbit pAb ABclonal A14079 IP, 1:500 HIF-1α Rabbit mAb CST #36169S WB, 1:1000 Hexokinase 2 Polyclonal antibody Proteintech 22029-1-AP WB, 1:3000 PKM2-specific Polyclonal antibody Proteintech 15822-1-AP WB, 1:3000 PGK1 Polyclonal antibody Proteintech 17811-1-AP WB, 1:3000 LDHA-Specific Polyclonal antibody Proteintech 19987-1-AP WB, 1:3000 β-Actin Mouse monoclonal Sigma A1978 WB, 1:4000 α-Tubulin Rabbit mAb CST #2125 WB, 1:3000 LC3B antibody Sigma L7543 WB, 1:2000 p62/SQSTM1 antibody Sigma P0067 WB, 1:3000 Monoclonal ANTI-FLAG® M2 Sigma F1804 IP, 1:1000 LAMP2A Abcam ab125068 WB, 1:1000 LAMP1 Rabbit mAb CST #9091 WB, 1:3000 IF, 1:200 Hsc70 Polyclonal antibody Protientech 10654-1-AP WB, 1:3000 Anti-O-Linked N-Acetylglucosamine Rabbit mAb PTM BIO PTM-951RM IP, 1:1000 Galectin-3 Monoclonal antibody Protientech 60207-1-Ig IF, 1:200 MYC tag Polyclonal antibody Protientech 16286-1-AP IP, 1:1000 DDOST/OST48 Polyclonal antibody Protientech 14916-1-AP IP, 1:2000 STT3A Polyclonal antibody Protientech 12034-1-AP IP, 1:2000 DAD1 Polyclonal antibody Protientech 10531-1-AP IP, 1:2000 LRRK2 Rabbit pAb ABclonal A17253 WB, 1:2000 MEF2D Polyclonal antibody Protientech 14353-1-AP WB, 1:2000 Cathepsin B Polyclonal antibody Protientech 12216-1-AP WB, 1:2000 Cathepsin D Polyclonal antibody Protientech 55021-1-AP WB, 1:2000 TFEB Rabbit pAb ABclonal A7311 WB, 1:2000 Antibody Name (Secondary antibodies) Company Item number Application Peroxidase AffiniPure Goat Anti-Rabbit IgG Jackson ImmunoResearch 111-035-144 WB, 1:5000- 1:10000 Peroxidase AffiniPure Goat Anti-Mouse IgG Jackson ImmunoResearch 115-035-146 WB, 1:5000- 1:10000 Alexa Fluor®594-AffiniPure goat anti-rabbit IgG Jackson ImmunoResearch 115-585-146 IF, 1:200-400 Alexa Fluor®488-AffiniPure goat anti-mouse IgG Jackson ImmunoResearch 115-545-146 IF, 1:200-400 Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) CST #93702 IP, 1:2000 VeriBlot for IP Detection Reagent (HRP) Abcam ab131366 IP, 1:2000 Supplementary Table 2 qPCR primers sequences used in the present study. ..
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